Aanat (NM_012818) Rat Tagged ORF Clone
CAT#: RR205489
- TrueORF®
Aanat (Myc-DDK-tagged ORF) - Rat arylalkylamine N-acetyltransferase (Aanat), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_012818" in other vectors (3)
USD 198.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Aanat |
Synonyms | AA-NAT; Nat4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR205489 representing NM_012818
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGAGCATCCACCCCCTGAAACCTGAGGCCCTGCATCTGCCTCTTGGGACCTCAGAGTTCCTAGGCT GCCAGCGGCGCCACACACTCCCTGCCAGTGAGTTCCGCTGCCTCACGCCGGAGGATGCCACCAGTGCGTT TGAGATTGAGCGCGAAGCCTTTATCTCAGTCTCGGGTACCTGCCCCCTCCACTTGGATGAGATCCGGCAC TTCCTCACCCTGTGTCCAGAGCTGTCACTGGGCTGGTTCGAGGAGGGCTGTCTTGTGGCCTTCATCATCG GTTCACTTTGGGACAAGGAGAGACTTACTCAGGAGTCGCTGACACTACACAGGCCTGGAGGCCGCACGGC CCACCTGCACGTACTGGCCGTGCACCGAACCTTCCGGCAGCAGGGCAAGGGCTCCGTCCTGCTGTGGCGA TACCTTCACCACCTGGGGAGTCAGCCGGCGGTGCGCCGGGCTGTGCTCATGTGCGAGAACGCCCTGGTGC CCTTCTATGAGAAATTTGGTTTCCAGGCCATGGGCCCGTGCGCCATCACCATGGGATCTCTCACCTTCAC TGAGCTGCAATGTTCCTTACGGTGCCACACCTTCCTGAGGAGGAACAGTGGCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205489 representing NM_012818
Red=Cloning site Green=Tags(s) MLSIHPLKPEALHLPLGTSEFLGCQRRHTLPASEFRCLTPEDATSAFEIEREAFISVSGTCPLHLDEIRH FLTLCPELSLGWFEEGCLVAFIIGSLWDKERLTQESLTLHRPGGRTAHLHVLAVHRTFRQQGKGSVLLWR YLHHLGSQPAVRRAVLMCENALVPFYEKFGFQAMGPCAITMGSLTFTELQCSLRCHTFLRRNSGC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_012818 |
ORF Size | 615 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012818.2, NP_036950.1 |
RefSeq Size | 1311 bp |
RefSeq ORF | 618 bp |
Locus ID | 25120 |
UniProt ID | Q64666 |
Cytogenetics | 10q32.2 |
MW | 23.1 kDa |
Gene Summary | The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. [provided by RefSeq, Feb 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN205489 | Aanat (untagged ORF) - Rat arylalkylamine N-acetyltransferase (Aanat), (10 ug) |
USD 330.00 |
|
RR205489L3 | Lenti ORF clone of Aanat (Myc-DDK-tagged ORF) - Rat arylalkylamine N-acetyltransferase (Aanat), (10 ug) |
USD 630.00 |
|
RR205489L4 | Lenti ORF clone of Aanat (mGFP-tagged ORF) - Rat arylalkylamine N-acetyltransferase (Aanat), (10 ug) |
USD 630.00 |
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