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Home cDNA Clone TrueORF All Gnas ORF Clones

Gnas (NM_021845) Rat cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RR200466 Gnas (Myc-DDK-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 1, (10 ug), 10µg
$859
6-7 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RR200466L2 Lenti ORF clone of Gnas (mGFP-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 1, (10 ug)
$1350
$1100
9 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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TrueORF Data for RR200466
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Sequence Data: ORF Nucleotide Sequence
ORF Size: 3435 bp
Predicted Protein MW: kDa
Restriction Sites: SgfI-NotI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_021845.5, NP_068617
RefSeq Size: 3826 RefSeq ORF: 3435
Synonyms : Gnas1; Gnpas; Nesp55
LocusID: 24896 Cytogenetic: 3q42
Summary: This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene. [provided by RefSeq, Apr 2009].

 

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