CTLA4 (NM_005214) Human Recombinant Protein

CAT#: TP723874

Purified recombinant protein of Human cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 1

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human CTLA-4, the region of Ala37-Phe162, from gene Accession# NM_005214
• Tag: Fc chimera
• Predicted MW: 39.9 kDa
• Concentration: lot specific
• Purity: >95%, as determined by Coomassie stained SDS-PAGE
• Buffer: 1 x PBS
• Bioactivity: Recombinant human CTLA-4 inhibits the production of IL-2 induced by 0.5 μg/mL of CD80 on Jurkat cells in a dose-dependent manner. The ED50 for this effect 50 is 4 - 24 ng/mL.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_005205
• Locus ID: 1493
• UniProt ID: P16410
• Cytogenetics: 2q33.2
• Refseq Size: 1997
• Refseq ORF: 669
• Synonyms: ALPS5; CD; CD152; CELIAC3; CTLA-4; GRD4; GSE; IDDM12
• Summary: This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Autoimmune thyroid disease, Cell adhesion molecules (CAMs), T cell receptor signaling pathway