TP53 (NM_001126112) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC225604. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 43.5 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Stem cell - PluripotencyTranscription FactorsDruggable Genome Protein Pathways: MAPK signaling pathwayCell cyclep53 signaling pathwayApoptosisWnt signaling pathwayNeurotrophin signaling pathwayMore Pathways >>

Protein Data

RefSeq: NP_001119584	RefSeq Size:	RefSeq ORF: 1182
LocusID: 7157	Cytogenetic: 17p13.1
Synonyms : BCC7; LFS1; P53; TRP53
Summary: This gene encodes a tumor suppressor protein containing transcription activation, DNA-binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. The use of multiple promoters and alternative splicing results in multiple transcript variants, which can each be translated from one of two possible start codons, resulting in many isoforms for this gene. [provided by RefSeq, Feb 2013].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens tumor protein p53 (TP53), transcript variant 2 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens tumor protein p53 (TP53), transcript variant 2 as transfection-ready DNA • Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 2, mGFP tagged • Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 2 , Myc-DDK-tagged Related Lysate from OriGene • Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • TP53 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • TP53 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Trp53 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • TP53 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Trp53 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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