ENPP3 (NM_005021) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC224818. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 99.9 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: TransmembraneDruggable Genome Protein Pathways: Purine metabolismStarch and sucrose metabolismRiboflavin metabolismNicotinate and nicotinamide metabolismPantothenate and CoA biosynthesisMetabolic pathways

Protein Data

RefSeq: NP_005012	RefSeq Size: 2794	RefSeq ORF: 2628
LocusID: 5169	Cytogenetic: 6q22
Synonyms : B10; CD203c; NPP3; PD-IBETA; PDNP3
Summary: The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as transfection-ready DNA • Lenti ORF clone of Human ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) , Myc-DDK-tagged • Lenti ORF clone of Human ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), mGFP tagged Related Lysate from OriGene • Transient overexpression lysate of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) Validated Antibodies • CD203c Rabbit Polyclonal (Internal) Antibody • CD203c Rabbit Polyclonal (C-Terminus) Antibody • CD203c Rabbit Polyclonal (C-Terminus) Antibody • CD203c Rabbit Polyclonal (Internal) Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • ENPP3 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • ENPP3 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Enpp3 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • ENPP3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Enpp3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene ENPP3

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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