FADS2 (NM_004265) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC223780. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 52.1 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Transmembrane Protein Pathways: alpha-Linolenic acid metabolismBiosynthesis of unsaturated fatty acidsPPAR signaling pathway

Protein Data

RefSeq: NP_004256	RefSeq Size: 3149	RefSeq ORF: 1335
LocusID: 9415	Cytogenetic: 11q12.2
Synonyms : D6D; DES6; FADSD6; LLCDL2; SLL0262; TU13
Summary: The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens fatty acid desaturase 2 (FADS2) as transfection-ready DNA • Lenti ORF clone of Human fatty acid desaturase 2 (FADS2) , Myc-DDK-tagged • Lenti ORF clone of Human fatty acid desaturase 2 (FADS2), mGFP tagged • GFP-tagged ORF clone of Homo sapiens fatty acid desaturase 2 (FADS2) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of fatty acid desaturase 2 (FADS2) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • FADS2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • FADS2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Fads2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • FADS2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Fads2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene FADS2

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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