AADAT (NM_182662) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC222252. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 47.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways: Lysine biosynthesisLysine degradationTryptophan metabolismMetabolic pathways

Protein Data

RefSeq: NP_872603	RefSeq Size: 2108	RefSeq ORF: 1278
LocusID: 51166	Cytogenetic: 4q33
Synonyms : KAT2; KATII
Summary: This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens aminoadipate aminotransferase (AADAT), transcript variant 2 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens aminoadipate aminotransferase (AADAT), transcript variant 2 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of aminoadipate aminotransferase (AADAT), transcript variant 2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • AADAT - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • AADAT - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Aadat - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • AADAT - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Aadat - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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