HES5 (NM_001010926) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC215311. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 18 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: ES Cell Differentiation/IPSDruggable Genome Protein Pathways: Notch signaling pathway

Protein Data

RefSeq: NP_001010926	RefSeq Size: 1306	RefSeq ORF: 501
LocusID: 388585	Cytogenetic: 1p36.32
Synonyms : bHLHb38
Summary: This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer. [provided by RefSeq, Dec 2008].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens hairy and enhancer of split 5 (Drosophila) (HES5) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens hairy and enhancer of split 5 (Drosophila) (HES5) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of hairy and enhancer of split 5 (Drosophila) (HES5) Validated Antibodies • HES5 Rabbit Polyclonal (Internal) Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • HES5 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • HES5 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Hes5 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • HES5 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Hes5 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene HES5

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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