CCT2 (NM_006431) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC213873. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 57.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways:

Protein Data

RefSeq: NP_006422	RefSeq Size: 1962	RefSeq ORF: 1608
LocusID: 10576	Cytogenetic: 12q15
Synonyms : 99D8.1; CCT-beta; CCTB; PRO1633; TCP-1-beta
Summary: The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens chaperonin containing TCP1, subunit 2 (beta) (CCT2), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens chaperonin containing TCP1, subunit 2 (beta) (CCT2), transcript variant 1 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens chaperonin containing TCP1, subunit 2 (beta) (CCT2), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of chaperonin containing TCP1, subunit 2 (beta) (CCT2) Validated Antibodies • Rabbit monoclonal antibody against TCP1 beta(clone EPR4084) • Rabbit monoclonal antibody against TCP1 beta(clone EPR4083) • Rabbit Polyclonal antibody to TCP1 beta (chaperonin containing TCP1, subunit 2 (beta)) • TCP1 Beta / CCT2 Mouse Monoclonal (F39 P7 F11) Antibody • TCP1 Beta / CCT2 Rat anti-Mouse Monoclonal (PK/8/4/4i/2f) Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CCT2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CCT2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Cct2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CCT2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Cct2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CCT2

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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