POU5F1 (NM_002701) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC211998. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 38.4 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Stem cell - PluripotencyAdult stem cellsCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cellsTranscription Factors Protein Pathways:

Protein Data

RefSeq: NP_002692	RefSeq Size: 1417	RefSeq ORF: 1083
LocusID: 5460	Cytogenetic: 6p21.31
Synonyms : Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Summary: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Mar 2010].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of POU class 5 homeobox 1 (POU5F1), transcript variant 1 Validated Antibodies • Purified POU5F1 mouse monoclonal antibody,clone 2B6 • Purified POU5F1 mouse monoclonal antibody,clone 7H1 • Rabbit Polyclonal Antibody against OCT4 - Embryonic Stem Cell Marker • Rabbit monoclonal antibody against Oct-4(clone EPR2054) • Rabbit monoclonal antibody against Oct-4A(clone EPR2053(2)) • Rabbit Polyclonal POU5F1 Antibody • Purified Goat Polyclonal Antibody against OCT4 / POU5F1 • Purified OCT4 mouse monoclonal antibody, clone 9B7 • Carrier-free (BSA/glycerol-free) POU5F1 mouse monoclonal antibody,clone 2B6 • Carrier-free (BSA/glycerol-free) OCT4 mouse monoclonal antibody, clone 9B7 • Carrier-free (BSA/glycerol-free) POU5F1 mouse monoclonal antibody,clone 7H1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • POU5F1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • POU5F1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Pou5f1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • POU5F1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Pou5f1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene POU5F1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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