PTGIS (NM_000961) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC211030. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 56.9 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: TransmembraneP450Druggable Genome Protein Pathways: Arachidonic acid metabolismMetabolic pathways

Protein Data

RefSeq: NP_000952	RefSeq Size: 5603	RefSeq ORF: 1503
LocusID: 5740	Cytogenetic: 20q13.13
Synonyms : CYP8; CYP8A1; PGIS; PTGI
Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens prostaglandin I2 (prostacyclin) synthase (PTGIS) as transfection-ready DNA • Lenti ORF clone of Human prostaglandin I2 (prostacyclin) synthase (PTGIS) , Myc-DDK-tagged • Lenti ORF clone of Human prostaglandin I2 (prostacyclin) synthase (PTGIS), mGFP tagged • GFP-tagged ORF clone of Homo sapiens prostaglandin I2 (prostacyclin) synthase (PTGIS) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens prostaglandin I2 (prostacyclin) synthase (PTGIS) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of prostaglandin I2 (prostacyclin) synthase (PTGIS) Validated Antibodies • Rabbit polyclonal anti-PTGIS antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • PTGIS - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • PTGIS - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ptgis - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • PTGIS - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ptgis - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene PTGIS

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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