SPP1 (NM_001040060) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC210592. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 30.6 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Secreted ProteinDruggable Genome Protein Pathways: Focal adhesionECM-receptor interactionToll-like receptor signaling pathway

Protein Data

RefSeq: NP_001035149	RefSeq Size: 1560	RefSeq ORF: 864
LocusID: 6696	Cytogenetic: 4q22.1
Synonyms : BNSP; BSPI; ETA-1; OPN
Summary: The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 3 as transfection-ready DNA • Lenti ORF clone of Human secreted phosphoprotein 1 (SPP1), transcript variant 3, mGFP tagged • GFP-tagged ORF clone of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 3 as transfection-ready DNA • Lenti ORF clone of Human secreted phosphoprotein 1 (SPP1), transcript variant 3 , Myc-DDK-tagged Related Lysate from OriGene • Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 3 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • SPP1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • SPP1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Spp1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • SPP1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Spp1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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