MAPK1 (NM_002745) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC210493. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 41.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein KinaseDruggable Genome Protein Pathways: MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayOocyte meiosismTOR signaling pathwayVascular smooth muscle contractionMore Pathways >>

Protein Data

RefSeq: NP_002736	RefSeq Size: 5916	RefSeq ORF: 1083
LocusID: 5594	Cytogenetic: 22q11.21
Synonyms : ERK; ERK2; ERT1; MAPK2; p38; p40; p41; p41mapk; P42MAPK; PRKM1; PRKM2
Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human mitogen-activated protein kinase 1 (MAPK1), transcript variant 1, mGFP tagged • Lenti ORF clone of Human mitogen-activated protein kinase 1 (MAPK1), transcript variant 1 , Myc-DDK-tagged • GFP-tagged ORF clone of Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of mitogen-activated protein kinase 1 (MAPK1), transcript variant 1 Validated Antibodies • Purified Goat Polyclonal Antibody against MAPK1 • Rabbit Anti-ERK/MAPK (Thr202/Tyr204) Antibody (Phospho-Specific) • Rabbit monoclonal antibody against ERK2 p42 MAPK (C-term)(E460) • Rabbit Monoclonal antibody against MARK1, pT215 (Phospho-specific) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • MAPK1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • MAPK1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Mapk1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • MAPK1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Mapk1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene MAPK1

If you would like OriGene Technologies to prepare a purified protein in the quantity of your own interest, simply fill out the form and click Submit. OriGene will contact you.

Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
* Quantity (µg)
Comments
* First Name
* Last Name
* Email Address
* Institution Name
* Country
* Phone