CA8 (NM_004056) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC210228. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 32.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Nitrogen metabolism

Protein Data

RefSeq: NP_004047	RefSeq Size: 2278	RefSeq ORF: 873
LocusID: 767	Cytogenetic: 8q12.1
Synonyms : CA-VIII; CALS; CAMRQ3; CARP
Summary: The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens carbonic anhydrase VIII (CA8) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens carbonic anhydrase VIII (CA8) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of carbonic anhydrase VIII (CA8) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CA8 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CA8 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Car8 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CA8 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Car8 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CA8

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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