LEP (NM_000230) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC209259. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 16 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Secreted ProteinDruggable Genome Protein Pathways: Cytokine-cytokine receptor interactionNeuroactive ligand-receptor interactionJak-STAT signaling pathwayAdipocytokine signaling pathway

Protein Data

RefSeq: NP_000221	RefSeq Size: 3426	RefSeq ORF: 504
LocusID: 3952	Cytogenetic: 7q31.3
Synonyms : LEPD; OB; OBS
Summary: This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens leptin (LEP) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens leptin (LEP) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens leptin (LEP) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of leptin (LEP) Validated Antibodies • Anti-Leptin Mouse Monoclonal Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • LEP - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • LEP - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Lep - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • LEP - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Lep - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene LEP

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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