|Expression cDNA Clone and Sequence
Recombinant protein was produced with TrueORF clone, RC208749. Click on the TrueORF clone link to view cDNA and protein sequences.|
||Predicted MW:||47.9 kDa|
|Purity:||> 80% as determined by SDS-PAGE and Coomassie blue staining|
|Concentration:||>50 ug/mL as determined by microplate BCA method|
|Buffer and Storage:||25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.|
||Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
||SMAD3 Activity Verified in a DNA-binding Assay: SMAD3 (TP308749) activity was measured in a colorimetric DNA-binding assay. Purified SMAD3 protein containing a C-terminal MYC/DDK tag was incubated with biotinylated double-stranded oligonucleotide containing the SMAD3 consensus DNA-binding sequence. Following incubation, the reaction was transferred to a streptavidin-coated microplate to allow capture of the DNA-protein complex. After washing, the captured protein was detected with an anti-DDK peroxidase conjugate and colorimetric signal detection with TMB. Specificity of the protein-DNA interaction was confirmed by carrying out the binding in the presence of an unlabeled competitor oligonucleotide and by comparison to binding to an oligonucleotide containing a mutation in the consensus binding sequence.
||Stem cell relevant signaling - JAK/STAT signaling pathwayStem cell relevant signaling - TGFb/BMP signaling pathwayES Cell Differentiation/IPSCancer stem cellsEmbryonic stem cellsTranscription FactorsDruggable Genome
||Cell cycleWnt signaling pathwayTGF-beta signaling pathwayAdherens junctionPathways in cancerColorectal cancerMore Pathways >>