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Home Recombinant Human Proteins All SMAD3 Proteins

SMAD3 (NM_005902) Purified Human Protein

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Cat. No. Description Datasheet Price Availability*  
TP308749 Recombinant protein of human SMAD family member 3 (SMAD3), transcript variant 1, 20 ug (Larger pack size?) 680 In Stock
TA318074 SMAD3 Mouse Monoclonal (AF9F7) Antibody $345 3-7 Days
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OriGene Data
Species:Human Expression Host:HEK293 cells
Expression cDNA Clone or AA Sequence
Recombinant protein was produced with TrueORF clone, RC208749. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag:C-terminal MYC/DDK Predicted MW:47.9 kDa
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>50 ug/mL as determined by microplate BCA method
Buffer:25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
BioActivity: SMAD3 Activity Verified in a DNA-binding Assay: SMAD3 (TP308749) activity was measured in a colorimetric DNA-binding assay. Purified SMAD3 protein containing a C-terminal MYC/DDK tag was incubated with biotinylated double-stranded oligonucleotide containing the SMAD3 consensus DNA-binding sequence. Following incubation, the reaction was transferred to a streptavidin-coated microplate to allow capture of the DNA-protein complex. After washing, the captured protein was detected with an anti-DDK peroxidase conjugate and colorimetric signal detection with TMB. Specificity of the protein-DNA interaction was confirmed by carrying out the binding in the presence of an unlabeled competitor oligonucleotide and by comparison to binding to an oligonucleotide containing a mutation in the consensus binding sequence.
Protein Families: Stem cell relevant signaling - JAK/STAT signaling pathwayStem cell relevant signaling - TGFb/BMP signaling pathwayES Cell Differentiation/IPSCancer stem cellsEmbryonic stem cellsTranscription FactorsDruggable Genome
Protein Pathways: Cell cycleWnt signaling pathwayTGF-beta signaling pathwayAdherens junctionPathways in cancerColorectal cancerMore Pathways >>
Protein Data
Protein Image
BioActivity Data
BioActivity Image
 

Reference Data
RefSeq: NP_005893 RefSeq Size: 6256 RefSeq ORF: 1278
LocusID: 4088 Cytogenetic: 15q22.33  
Synonyms : HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3
Summary:
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

 

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