NFKB1 (NM_003998) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC208384. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 105.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Transcription FactorsDruggable Genome Protein Pathways: Metabolic pathwaysMAPK signaling pathwayChemokine signaling pathwayApoptosisToll-like receptor signaling pathwayNOD-like receptor signaling pathwayMore Pathways >>

Protein Data

RefSeq: NP_003989	RefSeq Size: 4104	RefSeq ORF: 2910
LocusID: 4790	Cytogenetic: 4q24
Synonyms : EBP-1; KBF1; NF-kappa-B; NF-kappaB; NF-kB1; NFkappaB; NFKB-p105; NFKB-p50; p105; p50
Summary: This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1), transcript variant 1 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1), transcript variant 1 Validated Antibodies • Rabbit monoclonal antibody against NF-kB p105/50 (E381) • Rabbit anti-NF-kB p105/p50 (Phospho-Ser337) polyclonal antibody (Phospho-specific) • Rabbit anti-NF-kB p105/p50 (Phospho-Ser893) polyclonal antibody (Phospho-specific) • Rabbit anti-NF-kB p105/p50 (Phospho-Ser907) polyclonal antibody (Phospho-specific) • NF-KappaB p50 Mouse Monoclonal (4D1) Antibody • Rabbit polyclonal NF-kB p105/p50 (Ab-932) antibody • Rabbit polyclonal NF-kB p105/p50 (Ab-337) antibody • Rabbit polyclonal NF-kappaB p105/p50 (Ab-893) antibody • Rabbit polyclonal NF-kB p105/p50(Ab-907) antibody • Rabbit polyclonal NF-kB p105/p50 antibody • Rabbit polyclonal NF-kB p105/p50 (Ser893) antibody(Phospho-specific) • Rabbit polyclonal NF-kB p105/p50 (phospho-Ser927) antibody • Rabbit polyclonal NF-kB p105/p50 (Ab-927) antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • NFKB1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • NFKB1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Nfkb1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • NFKB1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Nfkb1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene NFKB1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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