EIF4E (NM_001968) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC207333. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 24.9 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways: mTOR signaling pathwayInsulin signaling pathway

Protein Data

RefSeq: NP_001959	RefSeq Size: 2493	RefSeq ORF: 654
LocusID: 1977	Cytogenetic: 4q21
Synonyms : CBP; EIF4E1; EIF4EL1; EIF4F
Summary: The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1 , Myc-DDK-tagged • Lenti ORF clone of Human eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1, mGFP tagged • GFP-tagged ORF clone of Homo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1 Validated Antibodies • Rabbit Monoclonal Antibody against EIF4E (Clone Y448) • Rabbit Monoclonal Antibody against EIF4E (Clone Y449) • Rabbit Monoclonal Antibody against EIF4E (Clone EP2151Y) • Rabbit polyclonal elF4E (Ser209) antibody(Phospho-specific) • Rabbit polyclonal elF4E (Ab-209) antibody • EIF4E mouse monoclonal antibody, clone 6C3 • EIF4E mouse monoclonal antibody, clone 6C11 • EIF4E mouse monoclonal antibody, clone 9H1 • EIF4E mouse monoclonal antibody,clone 5D11 • Carrier-free (BSA/glycerol-free) EIF4E mouse monoclonal antibody, clone 6C3 • Carrier-free (BSA/glycerol-free) EIF4E mouse monoclonal antibody, clone 9H1 • Carrier-free (BSA/glycerol-free) EIF4E mouse monoclonal antibody,clone 5D11 • Carrier-free (BSA/glycerol-free) EIF4E mouse monoclonal antibody, clone 6C11 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • EIF4E - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • EIF4E - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Eif4e - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • EIF4E - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Eif4e - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene EIF4E

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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