IMPDH1 (NM_183243) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC207118. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 60.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Purine metabolismDrug metabolism - other enzymesMetabolic pathways

Protein Data

RefSeq: NP_899066	RefSeq Size: 2534	RefSeq ORF: 1692
LocusID: 3614	Cytogenetic: 7q31.3
Synonyms : IMPD; IMPD1; LCA11; RP10; sWSS2608
Summary: The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens IMP (inosine 5'-monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 2 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens IMP (inosine 5'-monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 2 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens IMP (inosine 5'-monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 2 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of IMP (inosine monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • IMPDH1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • IMPDH1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Impdh1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • IMPDH1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Impdh1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

If you would like OriGene Technologies to prepare a purified protein in the quantity of your own interest, simply fill out the form and click Submit. OriGene will contact you.

Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
* Quantity (µg)
Comments
* First Name
* Last Name
* Email Address
* Institution Name
* Country
* Phone