CA1 (NM_001738) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC206616. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 28.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Nitrogen metabolism

Protein Data

RefSeq: NP_001729	RefSeq Size: 1264	RefSeq ORF: 786
LocusID: 759	Cytogenetic: 8q21.2
Synonyms : CA-I; CAB; Car1
Summary: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature. [provided by RefSeq, Sep 2009].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens carbonic anhydrase I (CA1), transcript variant 2 as transfection-ready DNA • Lenti ORF clone of Human carbonic anhydrase I (CA1), transcript variant 2 , Myc-DDK-tagged • Lenti ORF clone of Human carbonic anhydrase I (CA1), transcript variant 2, mGFP tagged • GFP-tagged ORF clone of Homo sapiens carbonic anhydrase I (CA1), transcript variant 2 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens carbonic anhydrase I (CA1), transcript variant 2 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of carbonic anhydrase I (CA1), transcript variant 2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CA1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CA1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Car1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CA1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Car1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CA1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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