MAPK11 (NM_002751) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC206583. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 41.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein KinaseDruggable Genome Protein Pathways: MAPK signaling pathwayVEGF signaling pathwayToll-like receptor signaling pathwayNOD-like receptor signaling pathwayRIG-I-like receptor signaling pathwayT cell receptor signaling pathwayMore Pathways >>

Protein Data

RefSeq: NP_002742	RefSeq Size: 2420	RefSeq ORF: 1095
LocusID: 5600	Cytogenetic: 22q13.33
Synonyms : p38-2; P38B; p38Beta; P38BETA2; PRKM11; SAPK2; SAPK2B
Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. This kinase is most closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and environmental stress. This kinase is activated through its phosphorylation by MAP kinase kinases (MKKs), preferably by MKK6. Transcription factor ATF2/CREB2 has been shown to be a substrate of this kinase. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens mitogen-activated protein kinase 11 (MAPK11) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens mitogen-activated protein kinase 11 (MAPK11) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of mitogen-activated protein kinase 11 (MAPK11) Validated Antibodies • Purified anti-MAPK11 mouse monoclonal antibody, clone 1C2 • Carrier-free (BSA/glycerol-free) MAPK11 mouse monoclonal antibody, clone 1C2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • MAPK11 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • MAPK11 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Mapk11 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • MAPK11 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Mapk11 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene MAPK11

If you would like OriGene Technologies to prepare a purified protein in the quantity of your own interest, simply fill out the form and click Submit. OriGene will contact you.

Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
* Quantity (µg)
Comments
* First Name
* Last Name
* Email Address
* Institution Name
* Country
* Phone