STAT5A (NM_003152) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC205753. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 90.5 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Stem cell relevant signaling - DSL/Notch pathwayStem cell relevant signaling - JAK/STAT signaling pathwayES Cell Differentiation/IPSTranscription FactorsDruggable Genome Protein Pathways: ErbB signaling pathwayJak-STAT signaling pathwayPathways in cancerChronic myeloid leukemiaAcute myeloid leukemia

Protein Data

RefSeq: NP_003143	RefSeq Size: 4298	RefSeq ORF: 2385
LocusID: 6776	Cytogenetic: 17q11.2
Synonyms : MGF; STAT5
Summary: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens signal transducer and activator of transcription 5A (STAT5A) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens signal transducer and activator of transcription 5A (STAT5A) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of signal transducer and activator of transcription 5A (STAT5A) Validated Antibodies • Luminex validated purified STAT5A mouse monoclonal antibody (clone 10H1), capture • Elisa and Luminex validated purified STAT5A mouse monoclonal antibody (clone 8H1), capture • Luminex validated purified STAT5A mouse monoclonal antibody (clone 6D6), capture • Elisa and Luminex validated purified STAT5A mouse monoclonal antibody (clone 4H1), capture • ELISA and Luminex validated purified biotinylated STAT5A mouse monoclonal antibody (clone 4H1), detection • ELISA and Luminex validated purified biotinylated STAT5A mouse monoclonal antibody (clone 8H1), detection • Rabbit Monoclonal Antibody against STAT5A (Clone E208) • Rabbit Monoclonal Antibody against STAT5A (Clone E289) • Rabbit polyclonal STAT5A (Ab-780) antibody • Rabbit polyclonal STAT5A (Ab-694) antibody • Rabbit polyclonal anti-STAT5A/B antibody • Rabbit polyclonal anti-STAT5A antibody • Rabbit polyclonal anti-STAT5A antibody • Purified Stat5a mouse monoclonal antibody, clone 4H1 • Purified STAT5A mouse monoclonal antibody, clone 10H1 • Purified STAT5A mouse monoclonal antibody, clone 9F7 • Purified STAT5A mouse monoclonal antibody, clone 8H1 • Purified STAT5A mouse monoclonal antibody, clone 4E7 • Purified STAT5A mouse monoclonal antibody, clone 6D6 • Purified STAT5A mouse monoclonal antibody, clone 11A9 • Purified STAT5A mouse monoclonal antibody, clone 3D2 • Purified STAT5A mouse monoclonal antibody, clone 1H9 • Purified STAT5A mouse monoclonal antibody, clone 9A5 • Purified STAT5A mouse monoclonal antibody, clone 3F9 • Rabbit anti-STAT5A (Phospho-Tyr694) polyclonal antibody (Phospho-specific) • Rabbit anti-STAT5A (Phospho-Ser780) polyclonal antibody (Phospho-specific) • Carrier-free (BSA/glycerol-free) STAT5A mouse monoclonal antibody, clone 10H1 • Carrier-free (BSA/glycerol-free) STAT5A mouse monoclonal antibody, clone 9F7 • Carrier-free (BSA/glycerol-free) STAT5A mouse monoclonal antibody, clone 8H1 • Carrier-free (BSA/glycerol-free) STAT5A mouse monoclonal antibody, clone 4E7 • Carrier-free (BSA/glycerol-free) STAT5A mouse monoclonal antibody, clone 6D6 • Carrier-free (BSA/glycerol-free) STAT5A mouse monoclonal antibody, clone 11A9 • Carrier-free (BSA/glycerol-free) STAT5A mouse monoclonal antibody, clone 3D2 • Carrier-free (BSA/glycerol-free) STAT5A mouse monoclonal antibody, clone 1H9 • Carrier-free (BSA/glycerol-free) STAT5A mouse monoclonal antibody, clone 9A5 • Carrier-free (BSA/glycerol-free) Stat5a mouse monoclonal antibody, clone 4H1 • Carrier-free (BSA/glycerol-free) STAT5A mouse monoclonal antibody, clone 3F9 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • STAT5A - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • STAT5A - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Stat5a - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • STAT5A - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Stat5a - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene STAT5A

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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