ADH1B (NM_000668) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC205391. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 39.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Glycolysis / GluconeogenesisFatty acid metabolismTyrosine metabolismRetinol metabolismMetabolism of xenobiotics by cytochrome P450Drug metabolism - cytochrome P450More Pathways >>

Protein Data

RefSeq: NP_000659	RefSeq Size: 2666	RefSeq ORF: 1128
LocusID: 125	Cytogenetic: 4q23
Synonyms : ADH2
Summary: The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B) as transfection-ready DNA • Lenti ORF clone of Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B) , Myc-DDK-tagged • Lenti ORF clone of Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B), mGFP tagged • GFP-tagged ORF clone of Homo sapiens alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B) Validated Antibodies • Purified ADH1B mouse monoclonal antibody, clone 1C3 • Purified ADH1B mouse monoclonal antibody, clone 1H7 • Purified ADH1B mouse monoclonal antibody, clone 4F12 • Purified ADH1B mouse monoclonal antibody, clone 5D7 • Purified ADH1B mouse monoclonal antibody, clone 3C12 • Purified ADH1B mouse monoclonal antibody, clone 3F2 • Carrier-free (BSA/glycerol-free) ADH1B mouse monoclonal antibody, clone 1C3 • Carrier-free (BSA/glycerol-free) ADH1B mouse monoclonal antibody, clone 1H7 • Carrier-free (BSA/glycerol-free) ADH1B mouse monoclonal antibody, clone 4F12 • Carrier-free (BSA/glycerol-free) ADH1B mouse monoclonal antibody, clone 5D7 • Carrier-free (BSA/glycerol-free) ADH1B mouse monoclonal antibody, clone 3C12 • Carrier-free (BSA/glycerol-free) ADH1B mouse monoclonal antibody, clone 3F2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • ADH1B - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • ADH1B - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • ADH1B - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene ADH1B

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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