PRKCI (NM_002740) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC205379. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 68.1 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein KinaseDruggable Genome Protein Pathways: EndocytosisTight junctionInsulin signaling pathway

Protein Data

RefSeq: NP_002731	RefSeq Size: 4884	RefSeq ORF: 1791
LocusID: 5584	Cytogenetic: 3q26.3
Synonyms : DXS1179E; nPKC-iota; PKCI
Summary: This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens protein kinase C, iota (PRKCI) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens protein kinase C, iota (PRKCI) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens protein kinase C, iota (PRKCI) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of protein kinase C, iota (PRKCI) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • PRKCI - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • PRKCI - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Prkci - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • PRKCI - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Prkci - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene PRKCI

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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