TBK1 (NM_013254) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC205238. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 83.5 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein KinaseDruggable Genome Protein Pathways: Toll-like receptor signaling pathwayRIG-I-like receptor signaling pathwayCytosolic DNA-sensing pathway

Protein Data

RefSeq: NP_037386	RefSeq Size: 2982	RefSeq ORF: 2190
LocusID: 29110	Cytogenetic: 12q14.1
Synonyms : NAK; T2K
Summary: The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens TANK-binding kinase 1 (TBK1) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens TANK-binding kinase 1 (TBK1) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of TANK-binding kinase 1 (TBK1) Validated Antibodies • NAK / TBK1 Mouse Monoclonal (aa563-577) (108A429 ) Antibody • Rabbit Polyclonal NAK Antibody • NAK / TBK1 Mouse Monoclonal (aa175-188) (72B587) Antibody • Rabbit monoclonal antibody against NAK / TBK1(clone EPR2867(2)) • Rabbit monoclonal antibody against NAK/TBK1 Phospho (pS172)(clone EPR2867(2)) (Phospho-Specific) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • TBK1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • TBK1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Tbk1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • TBK1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Tbk1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene TBK1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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