CBLC (NM_012116) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC205130. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 52.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: ErbB signaling pathwayUbiquitin mediated proteolysisEndocytosisJak-STAT signaling pathwayT cell receptor signaling pathwayInsulin signaling pathwayMore Pathways >>

Protein Data

RefSeq: NP_036248	RefSeq Size: 1575	RefSeq ORF: 1425
LocusID: 23624	Cytogenetic: 19q13.2
Synonyms : CBL-3; CBL-SL; RNF57
Summary: This gene encodes a member of the Cbl family of E3 ubiquitin ligases. Cbl proteins play important roles in cell signaling through the ubiquitination and subsequent downregulation of tyrosine kinases. Expression of this gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 1 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 1 Validated Antibodies • Purified CBLC mouse monoclonal antibody,clone 3D9 • Purified CBLC mouse monoclonal antibody,clone 3B4 • Purified CBLC mouse monoclonal antibody,clone 4E3 • Carrier-free (BSA/glycerol-free) CBLC mouse monoclonal antibody,clone 3B4 • Carrier-free (BSA/glycerol-free) CBLC mouse monoclonal antibody,clone 4E3 • Carrier-free (BSA/glycerol-free) CBLC mouse monoclonal antibody,clone 3D9 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CBLC - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CBLC - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Cblc - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CBLC - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Cblc - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CBLC

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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