SPRY2 (NM_005842) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC204864. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 34.5 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Jak-STAT signaling pathway

Protein Data

RefSeq: NP_005833	RefSeq Size: 2126	RefSeq ORF: 948
LocusID: 10253	Cytogenetic: 13q31.1
Synonyms : hSPRY2
Summary: This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens sprouty homolog 2 (Drosophila) (SPRY2) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens sprouty homolog 2 (Drosophila) (SPRY2) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens sprouty homolog 2 (Drosophila) (SPRY2) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of sprouty homolog 2 (Drosophila) (SPRY2) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • SPRY2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • SPRY2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Spry2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • SPRY2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Spry2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene SPRY2

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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