HNF1B (NM_000458) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC204453. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 61.1 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: ES Cell Differentiation/IPSEmbryonic stem cellsTranscription FactorsDruggable Genome Protein Pathways: Maturity onset diabetes of the young

Protein Data

RefSeq: NP_000449	RefSeq Size: 2816	RefSeq ORF: 1674
LocusID: 6928	Cytogenetic: 17q12
Synonyms : FJHN; HNF-1B; HNF1beta; HNF2; HPC11; LF-B3; LFB3; MODY5; TCF-2; TCF2; VHNF1
Summary: This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens HNF1 homeobox B (HNF1B), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens HNF1 homeobox B (HNF1B), transcript variant 1 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens HNF1 homeobox B (HNF1B), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of HNF1 homeobox B (HNF1B), transcript variant 1 Validated Antibodies • Purified Goat Polyclonal Antibody against TCF2 / VHNF1 • Rabbit Monoclonal antibody against HNF 1 Beta (HNF1B) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • HNF1B - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • TCF2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Hnf1b - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • HNF1B - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Hnf1b - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene HNF1B

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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