CALML5 (NM_017422) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC204372. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 15.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways: Calcium signaling pathwayPhosphatidylinositol signaling systemOocyte meiosisVascular smooth muscle contractionLong-term potentiationNeurotrophin signaling pathwayMore Pathways >>

Protein Data

RefSeq: NP_059118	RefSeq Size: 854	RefSeq ORF: 441
LocusID: 51806	Cytogenetic: 10p15.1
Synonyms : CLSP
Summary: This gene encodes a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes. [provided by RefSeq, Jul 2008]. COMPLETENESS: complete on the 3' end.

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens calmodulin-like 5 (CALML5) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens calmodulin-like 5 (CALML5) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens calmodulin-like 5 (CALML5) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of calmodulin-like 5 (CALML5) Validated Antibodies • Rabbit polyclonal anti-CALML5 (CABP7) antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CALML5 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CALML5 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Calm4 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CALML5 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Calm4 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CALML5

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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