UPF3A (NM_080687) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC204333. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 50.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways:

Protein Data

RefSeq: NP_542418	RefSeq Size: 2282	RefSeq ORF: 1332
LocusID: 65110	Cytogenetic: 13q34
Synonyms : HUPF3A; RENT3A; UPF3
Summary: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens UPF3 regulator of nonsense transcripts homolog A (yeast) (UPF3A), transcript variant 2 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens UPF3 regulator of nonsense transcripts homolog A (yeast) (UPF3A), transcript variant 2 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of UPF3 regulator of nonsense transcripts homolog A (yeast) (UPF3A), transcript variant 2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • UPF3A - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • UPF3A - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Upf3a - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • UPF3A - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Upf3a - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

If you would like OriGene Technologies to prepare a purified protein in the quantity of your own interest, simply fill out the form and click Submit. OriGene will contact you.

Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
* Quantity (µg)
Comments
* First Name
* Last Name
* Email Address
* Institution Name
* Country
* Phone