CCL5 (NM_002985) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC203799. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 7.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Secreted ProteinTransmembraneDruggable Genome Protein Pathways: Cytokine-cytokine receptor interactionChemokine signaling pathwayToll-like receptor signaling pathwayNOD-like receptor signaling pathwayCytosolic DNA-sensing pathwayPrion diseasesMore Pathways >>

Protein Data

RefSeq: NP_002976	RefSeq Size: 1237	RefSeq ORF: 276
LocusID: 6352	Cytogenetic: 17q11.2
Synonyms : D17S136E; RANTES; SCYA5; SISd; TCP228
Summary: This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. [provided by RefSeq, Jul 2008].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens chemokine (C-C motif) ligand 5 (CCL5) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens chemokine (C-C motif) ligand 5 (CCL5) as transfection-ready DNA • Lenti ORF clone of Human chemokine (C-C motif) ligand 5 (CCL5) , Myc-DDK-tagged • Lenti ORF clone of Human chemokine (C-C motif) ligand 5 (CCL5), mGFP tagged Related Lysate from OriGene • Transient overexpression lysate of chemokine (C-C motif) ligand 5 (CCL5) Validated Antibodies • Rabbit monoclonal antibody against RANTES(clone EPR1359) • CCL5 / RANTES Mouse Monoclonal Antibody • Anti-CRP Mouse Monoclonal Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CCL5 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CCL5 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ccl5 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CCL5 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ccl5 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CCL5

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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