ARF6 (NM_001663) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC203600. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 19.9 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways: EndocytosisFc gamma R-mediated phagocytosis

Protein Data

RefSeq: NP_001654	RefSeq Size: 1806	RefSeq ORF: 528
LocusID: 382	Cytogenetic: 14q21.3
Synonyms : DKFZp564M0264
Summary: This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens ADP-ribosylation factor 6 (ARF6) as transfection-ready DNA • Lenti ORF clone of Human ADP-ribosylation factor 6 (ARF6) , Myc-DDK-tagged • Lenti ORF clone of Human ADP-ribosylation factor 6 (ARF6), mGFP tagged • GFP-tagged ORF clone of Homo sapiens ADP-ribosylation factor 6 (ARF6) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of ADP-ribosylation factor 6 (ARF6) Validated Antibodies • Rabbit Monoclonal antibody against ARF6 • Rabbit polyclonal anti-ARF6 antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • ARF6 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • ARF6 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Arf6 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • ARF6 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Arf6 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene ARF6

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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