DHCR24 (NM_014762) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC203507. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 57.6 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: TransmembraneStem cell - PluripotencyDruggable Genome Protein Pathways: Steroid biosynthesisMetabolic pathways

Protein Data

RefSeq: NP_055577	RefSeq Size: 4286	RefSeq ORF: 1551
LocusID: 1718	Cytogenetic: 1p32.3
Synonyms : DCE; Nbla03646; seladin-1; SELADIN1
Summary: This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens 24-dehydrocholesterol reductase (DHCR24) as transfection-ready DNA • Lenti ORF clone of Human 24-dehydrocholesterol reductase (DHCR24), mGFP tagged • GFP-tagged ORF clone of Homo sapiens 24-dehydrocholesterol reductase (DHCR24) as transfection-ready DNA • Lenti ORF clone of Human 24-dehydrocholesterol reductase (DHCR24) , Myc-DDK-tagged Related Lysate from OriGene • Transient overexpression lysate of 24-dehydrocholesterol reductase (DHCR24) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • DHCR24 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • DHCR24 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Dhcr24 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • DHCR24 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Dhcr24 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene DHCR24

If you would like OriGene Technologies to prepare a purified protein in the quantity of your own interest, simply fill out the form and click Submit. OriGene will contact you.

Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
* Quantity (µg)
Comments
* First Name
* Last Name
* Email Address
* Institution Name
* Country
* Phone