MAD2L1 (NM_002358) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC203273. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 23.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Cell cycleOocyte meiosisProgesterone-mediated oocyte maturation

Protein Data

RefSeq: NP_002349	RefSeq Size: 1390	RefSeq ORF: 618
LocusID: 4085	Cytogenetic: 4q27
Synonyms : HSMAD2; MAD2
Summary: MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1) as transfection-ready DNA • Lenti ORF clone of Human MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1) , Myc-DDK-tagged • Lenti ORF clone of Human MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1), mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1) Validated Antibodies • Goat Anti-MAD2L1 Antibody • Rabbit polyclonal antibody to MAD2 (MAD2 mitotic arrest deficient-like 1 (yeast)) • Rabbit Polyclonal antibody to Mad2L1 (MAD2 mitotic arrest deficient-like 1 (yeast)) • Rabbit polyclonal anti-MAD2L1 antibody • Purified MAD2L1 (MAD2) mouse monoclonal antibody, clone 4D2 • Carrier-free (BSA/glycerol-free) MAD2L1 mouse monoclonal antibody, clone 4D2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • MAD2L1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • MAD2L1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Mad2l1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • MAD2L1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Mad2l1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene MAD2L1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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