MMP9 (NM_004994) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC202872. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 78.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: ProteaseDruggable Genome Protein Pathways: Leukocyte transendothelial migrationPathways in cancerBladder cancer

Protein Data

RefSeq: NP_004985	RefSeq Size: 2387	RefSeq ORF: 2124
LocusID: 4318	Cytogenetic: 20q11.2
Synonyms : CLG4B; GELB; MANDP2; MMP-9
Summary: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9) as transfection-ready DNA • Lenti ORF clone of Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9), mGFP tagged • GFP-tagged ORF clone of Homo sapiens matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9) as transfection-ready DNA • Lenti ORF clone of Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9) , Myc-DDK-tagged Related Lysate from OriGene • Transient overexpression lysate of matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9) Validated Antibodies • Rabbit monoclonal antibody against MMP-9 (EP1255Y ) • Rabbit monoclonal antibody against MMP-9 (propeptide) (EP1328Y ) • Rabbit monoclonal antibody against MMP-9 (EP1254 ) • Rabbit polyclonal anti-MMP-9 antibody • Anti-MMP-9 Mouse Monoclonal Antibody • Anti-MMP-9 Mouse Monoclonal Antibody • MMP-9 Mouse Monoclonal (F11P2C3) Antibody • Rabbit polyclonal anti-MMP-9 antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • MMP9 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • MMP9 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Mmp9 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • MMP9 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Mmp9 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene MMP9

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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