CLIC3 (NM_004669) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC202726. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 26.5 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Ion Channels: OtherDruggable Genome Protein Pathways:

Protein Data

RefSeq: NP_004660	RefSeq Size: 813	RefSeq ORF: 711
LocusID: 9022	Cytogenetic: 9q34.3
Synonyms : OTTHUMP00000022630; chloride intracellular channel 3
Summary: Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 3 is a member of the p64 family and is predominantly localized in the nucleus and stimulates chloride ion channel activity. In addition, this protein may participate in cellular growth control, based on its association with ERK7, a member of the MAP kinase family. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens chloride intracellular channel 3 (CLIC3) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens chloride intracellular channel 3 (CLIC3) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of chloride intracellular channel 3 (CLIC3) Validated Antibodies • Rabbit polyclonal antibody to CLIC3 (chloride intracellular channel 3) • Rabbit Monoclonal antibody against CLIC3 • Rabbit polyclonal anti-CLIC3 antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CLIC3 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CLIC3 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Clic3 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CLIC3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Clic3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CLIC3

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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