NCK2 (NM_003581) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC202557. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 42.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: ErbB signaling pathwayAxon guidanceT cell receptor signaling pathwayPathogenic Escherichia coli infection

Protein Data

RefSeq: NP_003572	RefSeq Size: 2698	RefSeq ORF: 1143
LocusID: 8440	Cytogenetic: 2q12
Synonyms : GRB4; NCKbeta
Summary: This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human NCK adaptor protein 2 (NCK2), transcript variant 1 , Myc-DDK-tagged • Lenti ORF clone of Human NCK adaptor protein 2 (NCK2), transcript variant 1, mGFP tagged • GFP-tagged ORF clone of Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant 1 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of NCK adaptor protein 2 (NCK2), transcript variant 1 Validated Antibodies • Rabbit polyclonal anti-NCK2 antibody • Rabbit monoclonal antibody against NCK2(clone EPR3333) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • NCK2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • NCK2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Nck2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • NCK2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Nck2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene NCK2

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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