COX5B (NM_001862) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC202511. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 10.6 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways: Oxidative phosphorylationMetabolic pathwaysCardiac muscle contractionAlzheimer's diseaseParkinson's diseaseHuntington's disease

Protein Data

RefSeq: NP_001853	RefSeq Size: 523	RefSeq ORF: 390
LocusID: 1329	Cytogenetic: 2q11.2
Synonyms : COXVB
Summary: Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens cytochrome c oxidase subunit Vb (COX5B), nuclear gene encoding mitochondrial protein as transfection-ready DNA • Lenti ORF clone of Human cytochrome c oxidase subunit Vb (COX5B), nuclear gene encoding mitochondrial protein , Myc-DDK-tagged • Lenti ORF clone of Human cytochrome c oxidase subunit Vb (COX5B), nuclear gene encoding mitochondrial protein, mGFP tagged • GFP-tagged ORF clone of Homo sapiens cytochrome c oxidase subunit Vb (COX5B), nuclear gene encoding mitochondrial protein as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens cytochrome c oxidase subunit Vb (COX5B), nuclear gene encoding mitochondrial protein as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of cytochrome c oxidase subunit Vb (COX5B) Validated Antibodies • Rabbit Polyclonal antibody to COX5B (cytochrome c oxidase subunit Vb) • Rabbit polyclonal anti-COX5B antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • COX5B - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • COX5B - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Cox5b - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • COX5B - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Cox5b - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene COX5B

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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