PYCARD (NM_145183) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC202409. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: NOD-like receptor signaling pathwayCytosolic DNA-sensing pathway

Protein Data

RefSeq: NP_660184	RefSeq Size: 763	RefSeq ORF: 407
LocusID: 29108	Cytogenetic: 16p11.2
Synonyms : ASC, TMS1, CARD5, MGC10332;ASC; CARD5; MGC10332; TMS; TMS-1; TMS1; apoptosis-associated speck-like protein containing a CARD; caspase recruitment domain protein 5; target of methylation-induced silencing-1; PYD and CARD domain containing
Summary: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens PYD and CARD domain containing (PYCARD), transcript variant 3 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens PYD and CARD domain containing (PYCARD), transcript variant 3 as transfection-ready DNA • Lenti ORF clone of Human PYD and CARD domain containing (PYCARD), transcript variant 3 , Myc-DDK-tagged • Lenti ORF clone of Human PYD and CARD domain containing (PYCARD), transcript variant 3, mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens PYD and CARD domain containing (PYCARD), transcript variant 3 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of PYD and CARD domain containing (PYCARD), transcript variant 3 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • PYCARD - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • PYCARD - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Pycard - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • PYCARD - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Pycard - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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