ACO1 (NM_002197) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC201857. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 98.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Citrate cycle (TCA cycle)Glyoxylate and dicarboxylate metabolismMetabolic pathways

Protein Data

RefSeq: NP_002188	RefSeq Size: 3498	RefSeq ORF: 2670
LocusID: 48	Cytogenetic: 9p21.1
Synonyms : ACONS; IREB1; IREBP; IREBP1; IRP1
Summary: Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens aconitase 1, soluble (ACO1) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens aconitase 1, soluble (ACO1) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens aconitase 1, soluble (ACO1) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of aconitase 1, soluble (ACO1) Validated Antibodies • Rabbit Monoclonal antibody against ACO1 • Rabbit polyclonal anti-IREB1 / ACO1 antibody • Rabbit polyclonal anti-IREB1 / ACO1 antibody • Rabbit polyclonal IREB1 / ACO1 (Ser711) antibody(Phospho-specific) • Goat Anti-ACO1 / Aconitase 1 Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • ACO1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • ACO1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Aco1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • ACO1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Aco1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene ACO1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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