||Expression Host:||Human 293HEK cells
|Expression cDNA Clone and Sequence
Recombinant protein was produced with TrueORF clone, RC201618. Click on the TrueORF clone link to view cDNA and protein sequences.|
||Predicted MW:||47.7 kDa|
|Purity:||> 80% as determined by SDS-PAGE and Coomassie blue staining|
|Concentration:||>50 ug/mL as determined by microplate BCA method|
|Buffer and Storage:||25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.|
||Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
||GATA3 Activity Verified in a DNA-binding Assay: DNA binding activity of GATA3 as a function of protein concentration. Sensitivity to competitor oligonucleotide demonstrates specificity of protein-DNA binding.
||Stem cell relevant signaling - JAK/STAT signaling pathwayES Cell Differentiation/IPSAdult stem cellsTranscription Factors