NCBP2 (NM_007362) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC201519. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 17.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Spliceosome

Protein Data

RefSeq: NP_031388	RefSeq Size: 2120	RefSeq ORF: 471
LocusID: 22916	Cytogenetic: 3q29
Synonyms : CBC2; CBP20; NIP1; PIG55
Summary: The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens nuclear cap binding protein subunit 2, 20kDa (NCBP2), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens nuclear cap binding protein subunit 2, 20kDa (NCBP2), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human nuclear cap binding protein subunit 2, 20kDa (NCBP2), transcript variant 1 , Myc-DDK-tagged • Lenti ORF clone of Human nuclear cap binding protein subunit 2, 20kDa (NCBP2), transcript variant 1, mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens nuclear cap binding protein subunit 2, 20kDa (NCBP2), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of nuclear cap binding protein subunit 2, 20kDa (NCBP2), transcript variant 1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • NCBP2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • NCBP2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ncbp2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • NCBP2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ncbp2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene NCBP2

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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