HMGA1 (NM_002131) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC201458. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 10.5 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Stem cell relevant signaling - JAK/STAT signaling pathwayStem cell - PluripotencyTranscription FactorsDruggable Genome Protein Pathways:

Protein Data

RefSeq: NP_002122	RefSeq Size: 1887	RefSeq ORF: 291
LocusID: 3159	Cytogenetic: 6p21
Synonyms : HMG-R; HMGA1A; HMGIY
Summary: This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens high mobility group AT-hook 1 (HMGA1), transcript variant 2 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens high mobility group AT-hook 1 (HMGA1), transcript variant 2 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens high mobility group AT-hook 1 (HMGA1), transcript variant 2 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of high mobility group AT-hook 1 (HMGA1), transcript variant 2 Validated Antibodies • Goat Anti-HMGA1 (aa9-21) Antibody • Goat Anti-HMGA1 Antibody • Rabbit Monoclonal antibody against HMGA1 • HMGIY / HMGA1 Rabbit Polyclonal (aa1-50) Antibody • HMGIY / HMGA1 Goat Polyclonal (Internal) Antibody • Anti-Aldolase Mouse Monoclonal Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • HMGA1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • HMGA1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Hmga1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • HMGA1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Hmga1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

If you would like OriGene Technologies to prepare a purified protein in the quantity of your own interest, simply fill out the form and click Submit. OriGene will contact you.

Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
* Quantity (µg)
Comments
* First Name
* Last Name
* Email Address
* Institution Name
* Country
* Phone