DDC (NM_000790) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC201345. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 53.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Histidine metabolismTyrosine metabolismPhenylalanine metabolismTryptophan metabolismMetabolic pathways

Protein Data

RefSeq: NP_000781	RefSeq Size: 1975	RefSeq ORF: 1443
LocusID: 1644	Cytogenetic: 7p12.2
Synonyms : AADC
Summary: The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens dopa decarboxylase (aromatic L-amino acid decarboxylase) (DDC), transcript variant 2 as transfection-ready DNA • Lenti ORF clone of Human dopa decarboxylase (aromatic L-amino acid decarboxylase) (DDC), transcript variant 2, mGFP tagged • Lenti ORF clone of Human dopa decarboxylase (aromatic L-amino acid decarboxylase) (DDC), transcript variant 2 , Myc-DDK-tagged • GFP-tagged ORF clone of Homo sapiens dopa decarboxylase (aromatic L-amino acid decarboxylase) (DDC), transcript variant 2 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of dopa decarboxylase (aromatic L-amino acid decarboxylase) (DDC), transcript variant 2 Validated Antibodies • Sheep Anti-DOPA Decarboxylase, Bovine Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • DDC - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • DDC - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ddc - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • DDC - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ddc - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene DDC

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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