IVD (NM_002225) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC201077. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 46.5 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Valine, leucine and isoleucine degradationMetabolic pathways

Protein Data

RefSeq: NP_002216	RefSeq Size: 2216	RefSeq ORF: 1272
LocusID: 3712	Cytogenetic: 15q14
Synonyms : ACAD2
Summary: Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens isovaleryl-CoA dehydrogenase (IVD), nuclear gene encoding mitochondrial protein, transcript variant 1 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens isovaleryl-CoA dehydrogenase (IVD), nuclear gene encoding mitochondrial protein, transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens isovaleryl-CoA dehydrogenase (IVD), nuclear gene encoding mitochondrial protein, transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens isovaleryl-CoA dehydrogenase (IVD), nuclear gene encoding mitochondrial protein, transcript variant 1 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens isovaleryl-CoA dehydrogenase (IVD), nuclear gene encoding mitochondrial protein, transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of isovaleryl Coenzyme A dehydrogenase (IVD), nuclear gene encoding mitochondrial protein, transcript variant 1 • Transient overexpression lysate of isovaleryl-CoA dehydrogenase (IVD), nuclear gene encoding mitochondrial protein, transcript variant 1 Validated Antibodies • Purified anti-IVD mouse monoclonal antibody, clone 5A11 • Purified anti-IVD mouse monoclonal antibody, clone 1F1 • Rabbit Polyclonal antibody to IVD (isovaleryl Coenzyme A dehydrogenase) • Purified anti-IVD mouse monoclonal antibody, clone 1B10 • Carrier-free (BSA/glycerol-free) IVD mouse monoclonal antibody, clone 1B10 • Carrier-free (BSA/glycerol-free) IVD mouse monoclonal antibody, clone 5A11 • Carrier-free (BSA/glycerol-free) IVD mouse monoclonal antibody, clone 1F1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • IVD - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • IVD - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ivd - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • IVD - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ivd - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene IVD

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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