TUBB3 (NM_006086) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200755. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 50.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: ES Cell Differentiation/IPSDruggable Genome Protein Pathways: Gap junctionPathogenic Escherichia coli infection

Protein Data

RefSeq: NP_006077	RefSeq Size: 1736	RefSeq ORF: 1353
LocusID: 10381	Cytogenetic: 16q24.3
Synonyms : beta-4; CDCBM; CFEOM3A; TUBB4
Summary: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens tubulin, beta 3 (TUBB3), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens tubulin, beta 3 (TUBB3), transcript variant 1 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of tubulin, beta 3 (TUBB3) Validated Antibodies • Rabbit Monoclonal Antibody against TUBB3 (Clone EPR1568Y) • Rabbit polyclonal anti-Tubulin beta (TUBB3) antibody • Purified TUBB3 (beta III Tubulin) mouse monoclonal antibody, clone 5H2 • Rabbit Polyclonal antibody to beta Tubulin 3 (tubulin, beta 3) • Rabbit Polyclonal Antibody against TUBB3 • Rabbit Monoclonal Antibody against TUBB3 (Clone EP1331Y) • Rabbit Monoclonal Antibody against TUBB3 (Clone EP1569Y) • Carrier-free (BSA/glycerol-free) TUBB3 mouse monoclonal antibody, clone 5H2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • TUBB3 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • TUBB3 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Tubb3 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • TUBB3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Tubb3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene TUBB3

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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