PTPN6 (NM_080548) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200728. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 67.5 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: PhosphataseStem cell - PluripotencyDruggable Genome Protein Pathways: Adherens junctionJak-STAT signaling pathwayNatural killer cell mediated cytotoxicityT cell receptor signaling pathwayB cell receptor signaling pathway

Protein Data

RefSeq: NP_536858	RefSeq Size: 2234	RefSeq ORF: 1794
LocusID: 5777	Cytogenetic: 12p13
Synonyms : HCP; HCPH; HPTP1C; PTP-1C; SH-PTP1; SHP-1; SHP-1L; SHP1
Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens protein tyrosine phosphatase, non-receptor type 6 (PTPN6), transcript variant 2 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens protein tyrosine phosphatase, non-receptor type 6 (PTPN6), transcript variant 2 as transfection-ready DNA • Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 6 (PTPN6), transcript variant 2, mGFP tagged • Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 6 (PTPN6), transcript variant 2 , Myc-DDK-tagged Related Lysate from OriGene • Transient overexpression lysate of protein tyrosine phosphatase, non-receptor type 6 (PTPN6), transcript variant 2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • PTPN6 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • PTPN6 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ptpn6 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • PTPN6 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ptpn6 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene PTPN6

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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