DLD (NM_000108) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200639. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 50.1 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Glycolysis / GluconeogenesisCitrate cycle (TCA cycle)Glycine, serine and threonine metabolismValine, leucine and isoleucine degradationPyruvate metabolismMetabolic pathways

Protein Data

RefSeq: NP_000099	RefSeq Size: 3579	RefSeq ORF: 1530
LocusID: 1738	Cytogenetic: 7q31
Synonyms : DLDH; E3; GCSL; LAD; PHE3
Summary: This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens dihydrolipoamide dehydrogenase (DLD) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens dihydrolipoamide dehydrogenase (DLD) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens dihydrolipoamide dehydrogenase (DLD) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of dihydrolipoamide dehydrogenase (DLD) Validated Antibodies • Purified DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone 6D5 • Purified DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone 8A10 • Purified DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone 4E11 • Purified DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone 5G7 • Purified DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone 6G6 • Purified DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone 4A10 • Purified DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone 4D5 • Rabbit Monoclonal antibody against DLD • Carrier-free (BSA/glycerol-free) DLD mouse monoclonal antibody,clone 6D5 • Carrier-free (BSA/glycerol-free) DLD mouse monoclonal antibody,clone 8A10 • Carrier-free (BSA/glycerol-free) DLD mouse monoclonal antibody,clone 4E11 • Carrier-free (BSA/glycerol-free) DLD mouse monoclonal antibody,clone 5G7 • Carrier-free (BSA/glycerol-free) DLD mouse monoclonal antibody,clone 6G6 • Carrier-free (BSA/glycerol-free) DLD mouse monoclonal antibody,clone 4A10 • Carrier-free (BSA/glycerol-free) DLD mouse monoclonal antibody,clone 4D5 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • DLD - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • DLD - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Dld - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • DLD - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Dld - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene DLD

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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