PIR (NM_001018109) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200626. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 31.9 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Transcription Factors Protein Pathways:

Protein Data

RefSeq: NP_001018119	RefSeq Size: 1282	RefSeq ORF: 873
LocusID: 8544	Cytogenetic: Xp22.2
Synonyms : OTTHUMP00000022961; OTTHUMP00000022962; pirin; pirin (iron-binding nuclear protein)
Summary: This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens pirin (iron-binding nuclear protein) (PIR), transcript variant 2 as transfection-ready DNA • Lenti ORF clone of Human pirin (iron-binding nuclear protein) (PIR), transcript variant 2 , Myc-DDK-tagged • Lenti ORF clone of Human pirin (iron-binding nuclear protein) (PIR), transcript variant 2, mGFP tagged • GFP-tagged ORF clone of Homo sapiens pirin (iron-binding nuclear protein) (PIR), transcript variant 2 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens pirin (iron-binding nuclear protein) (PIR), transcript variant 2 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of pirin (iron-binding nuclear protein) (PIR), transcript variant 2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • PIR - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • PIR - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Pir - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • PIR - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Pir - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

If you would like OriGene Technologies to prepare a purified protein in the quantity of your own interest, simply fill out the form and click Submit. OriGene will contact you.

Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
* Quantity (µg)
Comments
* First Name
* Last Name
* Email Address
* Institution Name
* Country
* Phone