BCAT2 (NM_001190) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200625. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 41.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Valine, leucine and isoleucine degradationValine, leucine and isoleucine biosynthesisPantothenate and CoA biosynthesisMetabolic pathways

Protein Data

RefSeq: NP_001181	RefSeq Size: 1595	RefSeq ORF: 1179
LocusID: 587	Cytogenetic: 19q13
Synonyms : BCAM; BCATM; BCT2; PP18
Summary: This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens branched chain amino-acid transaminase 2, mitochondrial (BCAT2), nuclear gene encoding mitochondrial protein, transcript variant a as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens branched chain amino-acid transaminase 2, mitochondrial (BCAT2), nuclear gene encoding mitochondrial protein, transcript variant a as transfection-ready DNA • Lenti ORF clone of Human branched chain amino-acid transaminase 2, mitochondrial (BCAT2), nuclear gene encoding mitochondrial protein, transcript variant a , Myc-DDK-tagged • Lenti ORF clone of Human branched chain amino-acid transaminase 2, mitochondrial (BCAT2), nuclear gene encoding mitochondrial protein, transcript variant a, mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens branched chain amino-acid transaminase 2, mitochondrial (BCAT2), nuclear gene encoding mitochondrial protein, transcript variant a as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of branched chain aminotransferase 2, mitochondrial (BCAT2), nuclear gene encoding mitochondrial protein, transcript variant a Validated Antibodies • Rabbit Polyclonal antibody to BCAT2 (branched chain aminotransferase 2, mitochondrial) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • BCAT2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • BCAT2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Bcat2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • BCAT2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Bcat2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene BCAT2

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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